O trabalho "Mesenchymal bone marrow stem cells within polyglycolic acid tube observed in vivo after six weeks enhance facial nerve regeneration" foi publicado na revista Brain Research (Fator de Impacto= 2.728), uma revista internacional de destaque dedicada à Neurociência. Segundo a autora, Dra. Heloisa Juliana Zabeu Rossi Costa, a relevância do artigo consiste na observação da superioridade, para a regeneração do nervos facial, das células-tronco diferenciadas em Schwann-like em relação às células-tronco indiferenciadas, quando associadas a um meio de suporte favorável (matriz extracelular em tubo de ácido poliglicólico). Além disso, o estudo conseguiu identificar as células implantadas integradas ao tecido neural e funcionalmente ativas após 6 semanas, comprovação inédita na literatura, provando que a sobrevida prolongada dessas células influenciou o resultado funcional. O trabalho também conta com a participação dos otorrinolaringologistas Prof. Dr. Ricardo Ferreira Bento e da Dra. Raquel Salomone, além de co-autores do ICBUSP, IBUSP, INCOR e Depto de Cir Plástica da FMUSP.
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Brain Res. 2013 May 13;1510:10-21. doi: 10.1016/j.brainres.2013.03.025. Epub 2013 Mar 28.
Mesenchymal bone marrow stem cells within polyglycolic acid tube observed in vivo after six weeks enhance facial nerve regeneration.
Costa HJZR, Bento RF, Salomone R, Azzi-Nogueira D, Zanatta DB, Costa MP, Da Silva CF, Strauss BE, Haddad LA.
Source: Department of Otorhinolaryngology, University of São Paulo Medical School, São Paulo, Brazil.
Autografting is the gold-standard method for facial nerve repair with tissue loss. Its association with high-quality scaffolds and cell implants has disclosed distinct experimental outcomes. The aim of this study was to evaluate the functional and histological effects of bone marrow stem cells (BMSC) combined with polyglycolic acid tube (PGAt) in autografted rat facial nerves. After neurotmesis of the mandibular branch of the rat facial nerve, surgical repair consisted of nerve autografting (groups A-E) contained in pGAT (groups B-E), filled with basement membrane matrix (groups C-E) with undifferentiated BMSC (group D) or Schwann-like cells that had differentiated from BMSC (group E). Axon morphometrics and an objective compound muscle action potentials (CMAP) analysis were conducted. Immunofluorescence assays were carried out with Schwann cell marker S100 and anti-β-galactosidase to label exogenous cells. Six weeks after surgery, animals from either cell-containing group had mean CMAP amplitudes significantly higher than control groups. Differently from other groups, facial nerves with Schwann-like cell implants had mean axonal densities within reference values. This same group had the highest mean axonal diameter in distal segments. We observed expression of the reporter gene lacZ in nerve cells in the graft and distally from it in groups D and E. Group-E cells had lacZ coexpressed with S100. In conclusion, regeneration of the facial nerve was improved by BMSC within PGAt in rats, yet Schwann-like cells were associated with superior effects. Accordingly, groups D and E had BMSC integrated in neural tissue with maintenance of former cell phenotype for six weeks.